# Quantitative Imaging in Cell Biology, Volume 123

Quantitative Imaging in Cell Biology is Volume 123 of Methods in Cell Biology, a reference collection on quantitative optical fluorescence microscopy and image analysis. It combines foundational guidance and protocols with applications including co-localization, ratiometric imaging, molecule counting, super-resolution and light-sheet microscopy.

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## At a glance

- Explains how microscope, fluorophore and digital detector choices affect quantitative imaging data.
- Includes step-by-step protocols for capturing and analyzing quantitative images.
- Covers co-localization, ratiometric imaging and molecule counting, alongside super-resolution and light-sheet methods.
- Also addresses microscope maintenance and standardized samples for resolution measurement.

## Pros

- Connects foundational measurement concepts with practical image-capture and analysis protocols.
- Its application coverage ranges from established quantitative approaches to newer microscopy techniques.

## Specifications

### Publication
- Publisher: Academic Press
- Publication date: July 10, 2014
- Edition: 1st
- Language: English
- Print length: 588 pages
- ISBN-10: 0124201385
- ISBN-13: 978-0124201385
### Dimensions and installation
- Weight listed: 3.3 pounds
- Dimensions: 7.75 x 1.25 x 9.5 inches

## A good fit for

- Researchers and graduate-level readers working with quantitative fluorescence microscopy or cell-image analysis.
- Readers seeking protocol and methods coverage across microscopy setup, quantitative applications and newer imaging approaches.

## Less suited to

- Readers looking for a general cell biology introduction rather than a methods-focused reference.
- Readers who need a current survey of methods published after this volume’s 2014 publication date.

## Frequently asked questions

### What topics does this volume cover?
It covers quantitative optical fluorescence microscopy and image analysis, including co-localization, ratiometric imaging, molecule counting, super-resolution and light-sheet microscopy.
### Does it include practical protocols?
Yes. The listing describes step-by-step protocols for capturing and analyzing quantitative images, as well as practical chapters on microscope maintenance and standardized samples for resolution measurements.
### When was this edition published?
The listed publication date is July 10, 2014, and the edition is identified as the first.

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## Images

![Quantitative Imaging in Cell Biology, Volume 123 — 1](https://best-deals.pro/i/XO56EOX2BWmGZraYBtwF10KE9gMyVhDzL_QVmn9mCWOd-Sx0ZdWstaNIm_1SGRXv2DdD7BcoaCcx-klLD7LJEnFoT4CoInPVWLwgrBacS5zynOss3gnQ.jpg)